Heterologous Membrane Proteins Co©\Trigger E. coli Filamentat

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For IMP©\GFP (green fluorescence protein) fusion expression,the GFP encoding DNA was inserted in the previously constructed vector at the 3’ end of the IMP encoding construct. Theprimers used for the construction have been listed in Table S2,and the one©\step cloning (Vazyme, C115) and transformationwere performed.

E. coli BL21 cells were transformed with the corresponding expression plasmids or empty plasmids. Cells were cultivated at 37°Cin LB medium containing 50 μg/mL kanamycin to an OD600 of 0.6,different concentrations of isopropyl β©\D©\1©\thiogalactopyranoside(IPTG) was added for induction, respectively. The added volumeof 5 μL, 10 μL, 15 μL, and 20 μL of IPTG corresponds to theworking concentrations of 0.025 mM, 0.05 mM, 0.075 mM, and0.1 mM. The cells were incubated at 20°C for 20 h beforemicroscopy analysis or CFU counting
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